Workshop: Basic Concepts of Molecular Biology and Bioinformatics

As part of the 64th National conference of the Association of Physiologists and Pharmacologists of India at Manipal Academy of Higher Education, Manipal, a One-day pre-conference workshop will be conducted on basic concepts of molecular biology and bioinformatics.

Organizing team: Departments of Pharmacology – KMC and MMMC, Manipal Academy of Higher education, Manipal.

Date: 28.11.2018

Resource Personnel:

  1. Dr. Sri Lakshmi Bhavani G, Mrs. Rituparna Chakraborty and Mr Ujjal Bose – Molecular biology – Basic techniques
  2. Dr. Kshitish Acharya K – Basic techniques in Bioinformatics

 Duration of Workshop: Part 1: 3.5 hours, Part 2: 3 hours

 Importance of the workshop:

Recent developments in molecular biology techniques have introduced a new dimension to address the research questions faced by physiologists and pharmacologists.  Furthermore, translation of laboratory results to information relevant for developing treatment strategies or clinical diagnostic modalities, demand the need for knowledge in basic molecular biology techniques. It is very important for academicians, scientists and researchers addressing research questions in physiology and pharmacology to gain fundamental knowledge of molecular biology techniques in tandem with the knowledge to analyse genomics data computationally using bioinformatics tools. In view of the increasing importance of basic molecular biology techniques in clinical application, the departments of pharmacology – KMC and MMMC, Manipal Academy of Higher Education, Manipal is organizing a one-day pre-conference workshop in basic molecular techniques and bioinformatics as part of  APPICON 2018.

Target participants/learners:

Early career faculty members, scientists, doctoral and post-graduate students with special interest in research in molecular Biology and Bioinformatics. 

Workshop Details and Techniques covered

The aim of Part 1 of this workshop will cover basic techniques involving sample processing and handling for molecular biology experiments pertaining to the research questions in physiology and pharmacology.

Exposure to the following techniques involving the extraction and quantification of DNA, RNA and preparation of cDNA for gene expression assays. (Risk Assessment (RA) and SOP forms and handouts to be provided).

Part 1: Molecular biology – Basic concepts (MB Laboratory – Dept of Pharmacology, KMC)

  1. Extraction of genomic DNA (9:00 am – 9:45 am)
  • Blood (sample storage and preparation)
  • Cell line and tissue (includes homogenisation)
  • Estimation of genomic DNA.
  1. Extraction of total mRNA (9:50 am – 10:30 am)
  • Blood (sample storage and preparation)
  • Cell line and tissue
  • Estimation of extracted mRNA.
  • Integrity check for extracted mRNA.
  1. Reverse transcription of total mRNA to synthesise cDNA (10:45 am – 11:40 am)
  • Preparation of reagents and work area for cDNA synthesis.
  • Preparation of master mixes.
  • Denaturation of RNA and annealing with random primers.
  • Setting-up PCR cycles on Thermocycler.
  • Preparation of (+) Reverse Transcription (RT) and (–) RT samples.
  • PCR run for cDNA synthesis.
  1. Resolution of genomic DNA, RNA and PCR products by Agarose Gel Electrophoresis (AGE) (11:45 am – 12:30 pm).

The aim of Part 2 of this workshop will provide an introduction to basic techniques and platforms for computational analysis of genomic data generated through molecular biology experiments. Training will be provided to carry out data analysis using genomic tools to understand functional genomics.

 Part 2: Basic techniques in Bioinformatics analysis (Digilab, MMMC, MAHE)

  1. Sequence analysis for molecular biology experiments: BLAST & MSA (1:30 pm – 2:30 pm)
  2. Expression data analysis for individual genes of interest (2:45 pm – 4:30 pm)